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Diagnostics
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Diagnostics
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Internship opportunities
Please contact Dr. Villamor for internship opportunities in diagnostics and tissue culture technologies
The ACPC provides comprehensive testing services to the agricultural industry. Here is the fee structure:
- $275 set-up fee for fewer than 24 samples
- $88.00 per sample for the nucleic acid extraction, making cDNA (reverse transcription) and carrying out the PCR for the internal control reactions to ensure the quality of the nucleic acid is a suitable template for the PCR reaction, and $33.00 for each virus test completed. If a sample is tested for 10 viruses, the cost would be $275+$88+$33*10= $693. If 10 samples are tested for 10 viruses, the cost would be 275+[$88+(33*10)]*10=$4,455. If 40 samples were tested for 10 viruses the total cost per sample would be [$88+(33*10)]*40=$16,720.
- HTS testing using Illumina: $660/sample run including preparation, sequencing, and bioinformatics analyses.
- HTS testing using Illumina with validation of positives for pathogens of concern using PCR: $1100/sample
- HTS testing using MinION: $330/sample run including preparation, sequencing, bioinformatics analyses.
- HTS testing using MinION with validation of positives for pathogens of concern using PCR: $660/sample
- Breeding accessions – beyond those covered by NCPN funded personnel and reagents: Clean-up/testing: $8,800/clean-up round, Maintenance: $1,550/year
- Controlled Import Permit: $4,400 per cultivar assayed. This includes four high-throughput sequencing (HTS) runs using Illumina technology, followed by PCR validation of any detected pathogens of concern. The estimated release date is two years after receiving the material.
- Plants distribution: $330/plant or TC StarPack + shipping
The cost for a Virus Mimicking Artificial Positive Control (ViMAPC) is $4,000 when based on published primer sequences and $5,000 when primers are developed by ACPC personnel.
ViMAPC construction